Citation :

Vikrant, "Evolution of Transgenic Technology in Cereal Crops: a Review Approach," International Journal of Computer Trends and Technology (IJCTT), vol. 5, no. 3, pp. 17-26, 2015. Crossref, https://doi.org/10.14445/22490183/IJBTT-V12P604

Abstract

This review study reports a concise analysis of investigations on origin and progress of transgenic technology in major cereal crops which is the integral part of the cereals improvement programmes. Most genetic transformation approaches of cereal crops have been majorly restricted to rice, wheat, maize, barley and oats. Rice has been considered as a model system for transgenesis and also for many molecular genetic studies. Moreover, certain parameters; such as selection of target tissues for desired gene transfer, screening of competent genotypes for regeneration and transformation, modes of gene deliveries and use of suitable selection markers for screening of putative transformants have been considered as major influencing factors for the success of transgenic technology. Immature embryo in general has been proved definitely the best target tissue for cereal transformation, but the optimal size and stage of the embryos is greatly affected by vegetative and physiological status of the mother plant. Amongst various modes of gene transformation applied in cereals biotechnology; biolistic-mediated gene delivery has been used more frequently than Agrobacteriummediated transformation techniques. In addition to conventional plant breeding techniques, the genetic transformation of cereals with agronomically important target genes is required to improve nutritional quality and quantity of cereals particularly resistance to various stresses and diseases.

Keywords

Cereals, Selection Markers, Reporter gene, Agrobacterium, Biolistic, Transgenic.

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